OUTRIGGERS Out-and-down style outriggers, operated independently for precise leveling. Oversized diameter ball-bearing swing circle with external gear. Wet multi-disk internal brake is spring-applied, pressure-released. SWING SYSTEM Externally mounted, double-reduction planetary driven by hydraulic motor. Wire Rope – 380' (115,8 m) of 5/8" (16 mm) rotation resistant type. Wet multi-disc internal brake is spring-applied, pressure-released. Maximum theoretical bottom-layer line pull 14,500 lb (6 577 kg). HOIST Maximum theoretical line speed 453 fpm (138 mpm). Load Hook – 5-ton (4,5 mton) capacity hook with heavy-duty swivel and weight is provided for single-line operation. Working range from 10Þ below horizontal to 80Þ above. Boom Elevation – Double-acting hydraulic cylinder. Quick Reeve Boom Point – Three high-density nylon load sheaves mounted on heavy-duty roller bearings. at 82018 Manitex 35124 C Features 35 Ton (31,8 mton) Capacity 5-Section 124' (37,8 m) Proportional Boom 165' 11" (50,6 m) Maximum Tip Height 22', 1.25" (6,7 m) Out-and-Down Outriggers at Full Extension Intermediate and Fully Extended Outrigger Charts are Standard FirstUp for Optional Front Stabilizer Auto Retract 2-Speed Planetary Hoist with Grooved Drum and Negative Draft Flange Load Moment Indicator with Digital Display, CAN Bus, Overload Shutdown and Internal Boom Length Cable Radio ATB Rugged, Weatherproof Electrical System with Circuit Status LEDs Removable Boom Rest System Pressure Gauge Clamp-On Mounting Aeroquip STC (Snap to Connect) Hydraulic Fitting Manitex UPTime Comprehensive Support Specs BOOM Inverted T-cross section, 5-section telescoping type, extended and retracted proportionally by a double-acting hydraulic cylinder and cable-crowd system. Zhejiang Provincial Key Laboratory of Genetic & Developmental Disorders, Hangzhou 310058, China.CraneWorks, Inc. Zhejiang Laboratory for Systems & Precision Medicine, Zhejiang University Medical Center, Hangzhou 311121, China. Institute of Hematology, Zhejiang University & Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou 310058, China. Insititute of Translational Medicine, Zhejiang University, Hangzhou 310003, China. The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu 322000, China. Institute of Natural Sciences, Shanghai Jiao Tong University, Shanghai 200240, China. School of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai 200240, China. This error does not affect the conclusions of the article.ĭepartment of Cell Biology, and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China. ( J) Bar graph showing quantifications of transcriptional activity in daughter cells derived from three groups of mother cells with no, low and high transcription of LMNA gene, respectively. R 2 represents the coefficient of determination. Each dot represents a pair of the mother cell and one of its daughter cells, n = 90 pairs. ( I) Transcriptional activity of the mother cell and its daughter cells at LMNA loci was quantified to generate the scatter plot. ( H) Snapshots of the transcription activity of LMNA gene in HeLa cells at indicated time points through mitosis. ( G) Bar graph showing transcription states of two LMNA alleles in the same cell (case in A, n = 284 cells) or pairs of sister chromatids (case in D, n = 229 cells). Intensities of both transcription sites were plotted, with each dot representing a different pair of sister chromatids, n = 240 pairs. ( F) Sister chromatids of the same allele have correlated transcription activity. ( E) Line scan of intensity profiles showing dynamic labeling of DNA and nascent RNAs at corresponding sister chromatids pointed by arrows in (D). ( D) Representative images revealing three distinct transcriptional states of sister chromatid pairs identified by co-labeling of dCas9-GFP 14X and stdMCPtdTomato. ( B, C) Line scan of intensity profiles illustrating dynamic labeling of DNA (green) and nascent RNAs (red) of locus 1 (B) and locus 2 (C) indicated in (A). ( A) Fluorescent images of a cell showing DNA labeling of two LMNA alleles labeled by dCas9-GFP 14X and their transcriptional kinetics indicated by stdMCP-tdTomato at multiple time points. TriTag monitors allele-specific transcriptional bursting across the cell cycle.
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